Detection of noncovalent FKBP-FK506 and FKBP-Rapamycin complexes by capillary electrophoresis-mass spectrometry and capillary electrophoresis-tandem mass spectrometry.

摘要: The well known biospecific noncovalent receptor-ligand association complexes between the immunophilin FKBP and immunosuppressive drugs FK506 Rapamycin (RM) were investigated by on-line capillary electrophoresis-mass spectrometry (CE-MS) under selected ion monitoring (SIM) conditions CE-MS with tandem mass (CE-MS/MS) reaction (SRM) conditions. Solutions of hFKBP (33.3 µM) dissolved in 50 mM ammonium acetate at pH 7.5. Samples that contained 100 µM or RM also prepared same solution By using these aqueous neutral conditions, samples analyzed SIM SRM target separated CE spectrometer detection individual [hFKBP + 7HJ7+ as 7HJ7+. In an experiment where a mixture was presence FKBP, nine-to-one ratio current abundances observed reported literature from other studies. These results suggest CE-MS/MS may be yet another analytical method for studying interactions biologically important macromolecules physiological