Hepatitis B Surface Antigen Levels and Sequences of Natural Hepatitis B Virus Variants Influence the Assembly and Secretion of Hepatitis D Virus

摘要: Various domains of hepatitis B surface antigen (HBsAg) are essential for the assembly and secretion D virus (HDV). This study investigated influences levels sequences HBsAg naturally occurring HBV variants on HDV. Six (HBV)-producing plasmids (three genotype three C) six expression that expressed various were constructed from sera HDV-infected patients. These cotransfected with HDV 1, 2, or 4 into Huh-7 hepatoma cell line. Serum DNA correlated RNA outcomes chronic (CHD) The generally but not genotypes levels. Swapping residue mutagenesis experiments HBsAg-coding revealed Pro-62 in cytosolic domain-I affects 2 those 1. pre-S2 N-terminal deletion mutant adversely genotypes. In patients, serum Viremia poor outcomes. conclusion, influenced by amounts HBsAg. For an effective treatment CHD, reduction production addition to suppression replication might be crucial.