Immunohistochemical differentiation of hepatitis D virus genotypes.
作者： S Hsu
关键词: Genotype 、 Virus 、 Keyhole limpet hemocyanin 、 Antibody 、 Hepatitis D virus 、 Virology 、 Hepadnaviridae 、 Mass screening 、 Biology 、 Antigen
摘要: Determination of hepatitis D virus (HDV) genotypes is epidemiologically and clinically important. Phylogenic analysis based on sequencing multiple HDV strains isolated from sera patients not convenient for mass screening in routine laboratories. This study was designed to develop genotype-specific antibodies against delta antigen (HDAg) apply these immunohistochemical differentiation formalin-fixed, paraffin-embedded liver biopsies patients. Divergence the carboxyl-terminal 19 amino acids large HDAg between I II more than 70%. Peptides covering residues were conjugated keyhole limpet hemocyanin used immunization. The generated confirmed their specificity by binding type-specific HDAgs expressed DNA-transfected Huh-7 hepatoma cells. Liver 6 who had dominant genotype 33 stained with antibodies. accuracy 94.9%, agreement serum hepatic staining nearly perfect (κ = 0.83). In summary, can be as immunogens generate These proven useful biopsies.
sci-hub.se 参考文章(32)
M Chao, S Y Hsieh, J Taylor, Role of two forms of hepatitis delta virus antigen: evidence for a mechanism of self-limiting genome replication. Journal of Virology. ,vol. 64, pp. 5066- 5069 ,(1990) , 10.1128/JVI.64.10.5066-5069.1990
F Imazeki, M Omata, M Ohto, Heterogeneity and evolution rates of delta virus RNA sequences. Journal of Virology. ,vol. 64, pp. 5594- 5599 ,(1990) , 10.1128/JVI.64.11.5594-5599.1990
J C Wu, P J Chen, M Y Kuo, S D Lee, D S Chen, L P Ting, Production of hepatitis delta virus and suppression of helper hepatitis B virus in a human hepatoma cell line. Journal of Virology. ,vol. 65, pp. 1099- 1104 ,(1991) , 10.1128/JVI.65.3.1099-1104.1991
Takashi Yamane, Keiko Miyano, Kazuhisa Taketa, Jiro Sato, Hidekazu Nakabayashi, Growth of human hepatoma cell lines with differentiated functions in chemically defined medium Cancer Research. ,vol. 42, pp. 3858- 3863 ,(1982)
Jaw-Ching Wu, I-Ann Huang, Yi-Hsiang Huang, Jen-Yu Chen, I-Jane Sheen, Mixed genotypes infection with hepatitis D virus Journal of Medical Virology. ,vol. 57, pp. 64- 67 ,(1999) , 10.1002/(SICI)1096-9071(199901)57:1<64::AID-JMV9>3.0.CO;2-W
F. L. Chang, P. J. Chen, S. J. Tu, C. J. Wang, D. S. Chen, The large form of hepatitis delta antigen is crucial for assembly of hepatitis delta virus. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 88, pp. 8490- 8494 ,(1991) , 10.1073/PNAS.88.19.8490
J. L. Casey, T. L. Brown, E. J. Colan, F. S. Wignall, J. L. Gerin, A genotype of hepatitis D virus that occurs in northern South America. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 90, pp. 9016- 9020 ,(1993) , 10.1073/PNAS.90.19.9016
Mario Rizzetto, GIORGIO VERME, SERAFINO RECCHIA, FERRUCCIO BONINO, PATRIZIA FARCI, SARINO ARICÒ, RENATA CALZIA, ANTONIO PICCIOTTO, MASSIMO COLOMBO, HANS POPPER, Chronic Hepatitis in Carriers of Hepatitis B Surface Antigen, with Intrahepatic Expression of the Delta Antigen: An Active and Progressive Disease Unresponsive to Immunosuppressive Treatment Annals of Internal Medicine. ,vol. 98, pp. 437- 441 ,(1983) , 10.7326/0003-4819-98-4-437
Montserrat Cotrina, Maria Buti, Rosendo Jardi, Josep Quer, Francisco Rodriguez, Carles Pascual, Rafael Esteban, Jaime Guardia, Hepatitis delta genotypes in chronic delta infection in the northeast of Spain (Catalonia) Journal of Hepatology. ,vol. 28, pp. 971- 977 ,(1998) , 10.1016/S0168-8278(98)80345-0
Jaw-Ching Wu, Chuan-Mu Chen, I-Jane Sheen, Shou-Dong Lee, Huey-Miin Tzeng, Kong-Bung Choo, Evidence of transmission of hepatitis D virus to spouses from sequence analysis of the viral genome Hepatology. ,vol. 22, pp. 1656- 1660 ,(1995) , 10.1002/HEP.1840220607