Proteomic analysis of the Actinidia deliciosa leaf apoplast during biotrophic colonization by Pseudomonas syringae pv. actinidiae

摘要: Abstract For plant pathogenic bacteria, adaptation to the apoplast is considered as key in establishment of parasitic lifestyle. Pseudomonas syringae pv. actinidiae (Psa), causal agent bacterial canker kiwifruit, uses leaves entry site colonize plants. Through a combined approach based on 2-DE, nanoLC-ESI-LIT-MS/MS and quantitative PCR, we investigated Psa colonization Actinidia deliciosa “Hayward” leaf during biotrophic phase. A total 58 differentially represented protein species were identified artificially inoculated leaves. Although pathogen increased its population density initial period colonization, defense mechanisms able impede further disease development. We concerted action different proteins mainly belonging metabolism category, which intervened at times participated reducing population. On other hand, BamA was highly first week whereas OmpA Cpn60 induced later. In addition presenting proteomic information molecular factors actively participating this pathosystem, our data characterize early events host will promote eventual development novel bioassays for detection kiwiplants. Biological significance This original study evaluates perspective interaction occurring into between specific actinidiae. Despite multiplication, blocked infection 21 days revealed by number differentially-represented artificially-inoculated control Three also recognized among over-represented molecules infected may contribute improve breeding programs aimed selecting resistant/tolerant kiwifruit cultivars toward P. actinidiae, present high representation here shown be involved resistance mechanisms. additional players facilitate technological future