Dendrimer phthalocyanine-encapsulated polymeric micelle-mediated photochemical internalization extends the efficacy of photodynamic therapy and overcomes drug-resistance in vivo

摘要: Clinically, the efficacy of chemotherapeutic agents can be dramatically reduced in cancer cells with multiple drug resistance (MDR). In doxorubicin-resistant breast cells, drugs accumulate only within discrete cytosolic organelles that abrogate their therapeutic effects vitro and vivo. Photochemical internalization (PCI), a specific branch photodynamic therapy (PDT), is novel strategy utilized for site-specific triggered drug/gene release. The objective this study was to evaluate nanoparticle-based PDT/PCI on reversal resistance. Dendrimer phthalocyanine-encapsulated polymeric micelle (DPc/m)-mediated PCI, combined doxorubicin, studied drug-resistant MCF-7 xenograft model. Our results show internalized DPc/m showed unique PCI properties inside thereby facilitating doxorubicin release from endo-lysosomes nuclei after photoirradiation. Moreover, ‘light before’ highest antitumor depth proliferating cell nuclear antigen-negative area tumor sections DPc/m-mediated PDT obviously increased by combination doxorubicin; indicates limitation light penetration PDT, which may improved PCI. We conclude nanotechnology-based possesses several clinical benefits, such as overcoming treating deeper lesions are intractable alone.