Optimization of solid state fermentation conditions for the production of cellulase by Trichoderma reesei.

作者: Durgesh Pratap , Devendra P Maurya , Jitendra P Maurya , Dhananjay Singh

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摘要: Cellulase production studies have been carried out using the fungal strain Trichoderma reesei NCIM 992 by three different lignocellulosic materials solid state fermentation (SSF). The effect of basic parameters (pH, temperature, moisture content, particle size substrate and moistening agent) on enzyme was studied. Maximum cellulase 2.63 U ml(-1) wheat bran as substrate. optimal conditions forcellulase forwheat were found to be: initial content - 70%, medium pH-5.0, temperature-30 degrees C, agents (MSS) (500 microm).The incubation time for six days. Results indicate scope further optimization obtain higher titres under SSF.

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