Lifetime-resolved assay procedures

摘要: Improved luminescent lifetime-resolved association assay techniques for detection of analytes in samples using two photophore-labelled probes, the photophores which have different emissive lifetimes. One is excitable by a modulated energy source to an excited state from may be transferred other photophore when close poximity thereto resulting excitation and emission photophore. Methods according invention involve associating first probe with analyte second or reaction mixture bringing sufficient proximity allow transfer occur. The formed, monitored at time beyond lifetime shorter-lived