Colorimetric resonant reflection as a direct biochemical assay technique

摘要: Abstract A novel approach for the detection of molecular interactions is presented in which a colorimetric resonant diffractive grating surface used as binding platform. guided mode phenomenon to produce an optical structure that, when illuminated with white light, designed reflect only single wavelength. When molecules are attached surface, reflected wavelength (color) shifted due change path light that coupled into grating. By linking receptor complementary can be detected without use any kind fluorescent probe or particle label. The technique capable resolving changes ∼0.1 nm thickness protein binding, and performed either immersed fluid dried. readout system consists lamp illuminates small spot at normal incidence through fiber optic probe, spectrometer collects second fiber, also incidence. reading several milliseconds, thus it possible quickly measure large number taking place parallel upon monitor reaction kinetics real time. It expected this technology will most useful applications where numbers biomolecular measured parallel, particularly labels alter inhibit functionality under study. High throughput screening pharmaceutical compound libraries targets, microarray protein–protein proteomics examples require sensitivity afforded by approach. In paper, we describe theory behind sensor, sensor fabrication approach, performance using example assays.