Localized diacylglycerol drives the polarization of the microtubule-organizing center in T cells.

摘要: The reorientation of the T cell microtubule-organizing center (MTOC) toward antigen-presenting enables directional secretion cytokines and lytic factors. By single-cell photoactivation antigen receptor, we show that MTOC polarization is driven by localized accumulation diacylglycerol (DAG). was closely preceded first production DAG then recruitment microtubule motor protein dynein. Blocking or disrupting localization impaired recruitment. Localized also required for cytotoxic cell-mediated killing. Furthermore, itself sufficient to induce transient polarization. Our data identify a DAG-dependent pathway signals through dynein control polarity in cells.