Serum microRNA profiles as prognostic or predictive markers in the multimodality treatment of patients with gastric cancer.

摘要: Despite the implementation of multimodality treatment strategies, persistently poor prognosis gastric cancer patients is predominantly caused by lack predictive markers for response assessment in neoadjuvant setting, preventing individualized therapy. Therefore, identification novel and prognostic application required. The aim present study was to characterize serum microRNA (miRNA/miR) profile undergoing therapy identify possible markers. consisted 32 with who had undergone either primary surgical resection (n=14) or followed (n=18). Histopathological regression defined as a major histopathological when resected specimens contained <10% vital residual tumor cells. Intratumoral miRNA isolated from pre-operative post-neoadjuvant blood samples. Initially, microarray analyses were performed six that received (three responders versus three non-responders), assess amplification dysregulated miRNAs. Based on these findings, validated all performing single reverse transcription-polymerase chain reaction (RT-PCR) analysis. Depending extent regression, differential expression identified analyses. profile, miR-21, miR-29a miR-221 selected additional validation. However, RT-PCR measurements miRNAs did not exhibit any value treated resection. Thus, current pilot failed using measurements, however, results revealed depending regression. findings may have been affected small sample size.