New assay for the measurement of selenoprotein P as a sepsis biomarker from serum.

摘要: Abstract Selenium (Se) is incorporated into selenoproteins as the 21st proteinogenic amino acid selenocysteine. Serum Se concentrations decline during critical illness and are indicative of poor prognosis. mainly contained in hepatically derived selenoprotein P (SePP) which controls expression antioxidative selenoproteins. Here, we describe development an immunoluminometric sandwich assay that uses two polyclonal sheep antihuman SePP antibodies. After assessing stability analyte, determined samples from healthy individuals patients with sepsis. The analytical detection limit was 0.016 mg SePP/L serum. linear on dilution. stable serum at room temperature for least 24 h resistant to six freeze-thaw cycles. Median concentration 3.04 mg SePP/L (25th–75th percentiles, 2.6–3.4 mg/L) corresponded 98.4 μg Se/L interlaboratory CV 0.06 mg/L. There no association gender, but differed between young older individuals. were significantly ( n =60) compared =318). Since contains major fraction Se, conclude downregulation biosynthesis or removal circulating blood underlies negative acute phase response illness.