Probing small-molecule microarrays with tagged proteins in cell lysates.
作者： Marius S. Pop , Dina Wassaf , Angela N. Koehler
DOI: 10.1002/9780470559277.CH140101
关键词:
摘要: The technique of small-molecule microarray (SMM) screening is based on the ability small molecules to bind various soluble proteins. This type interaction easily detected by presence a fluorescence signal produced labeled antibodies that specifically recognize unique sequence (tag) present target protein. fluorescent intensity values are determined signal-to-noise ratios (SNRs). SMM high-throughput, unbiased method can rapidly identify novel direct ligands for protein targets. binding-based assay format generally applicable most proteins, but it especially useful targets do not possess an enzymatic activity. SMMs enable in purified form or context cellular lysate, likely providing more physiologically relevant environment.
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sci-hub.st 参考文章(13)
James E Bradner, Olivia M McPherson, Angela N Koehler, A method for the covalent capture and screening of diverse small molecules in a microarray format. Nature Protocols. ,vol. 1, pp. 2344- 2352 ,(2006) , 10.1038/NPROT.2006.282
Isao Miyazaki, Siro Simizu, Hideo Okumura, Satoshi Takagi, Hiroyuki Osada, A small-molecule inhibitor shows that pirin regulates migration of melanoma cells Nature Chemical Biology. ,vol. 6, pp. 667- 673 ,(2010) , 10.1038/NCHEMBIO.423
Mahesh Uttamchandani, Daniel P. Walsh, Sonya M. Khersonsky, Xuan Huang, Shao Q. Yao, Young-Tae Chang, Microarrays of Tagged Combinatorial Triazine Libraries in the Discovery of Small-Molecule Ligands of Human IgG ACS Combinatorial Science. ,vol. 6, pp. 862- 868 ,(2004) , 10.1021/CC049900S
Benjamin Z Stanton, Lee F Peng, Nicole Maloof, Kazuo Nakai, Xiang Wang, Jay L Duffner, Kennedy M Taveras, Joel M Hyman, Sam W Lee, Angela N Koehler, James K Chen, Julia L Fox, Anna Mandinova, Stuart L Schreiber, A small molecule that binds Hedgehog and blocks its signaling in human cells Nature Chemical Biology. ,vol. 5, pp. 154- 156 ,(2009) , 10.1038/NCHEMBIO.142
Jiyoung A Hong, Dylan V Neel, Dina Wassaf, Francisco Caballero, Angela N Koehler, Recent discoveries and applications involving small-molecule microarrays Current Opinion in Chemical Biology. ,vol. 18, pp. 21- 28 ,(2014) , 10.1016/J.CBPA.2013.09.020
P. A. Clemons, N. E. Bodycombe, H. A. Carrinski, J. A. Wilson, A. F. Shamji, B. K. Wagner, A. N. Koehler, S. L. Schreiber, Small molecules of different origins have distinct distributions of structural complexity that correlate with protein-binding profiles Proceedings of the National Academy of Sciences of the United States of America. ,vol. 107, pp. 18787- 18792 ,(2010) , 10.1073/PNAS.1012741107
Marius S. Pop, Nicolas Stransky, Colin W. Garvie, Jean-Philippe Theurillat, Emily C. Hartman, Timothy A. Lewis, Cheng Zhong, Elizabeth K. Culyba, Fallon Lin, Douglas S. Daniels, Raymond Pagliarini, Lucienne Ronco, Angela N. Koehler, Levi A. Garraway, A Small Molecule That Binds and Inhibits the ETV1 Transcription Factor Oncoprotein Molecular Cancer Therapeutics. ,vol. 13, pp. 1492- 1502 ,(2014) , 10.1158/1535-7163.MCT-13-0689
D. Walsh, Y. Chang, Recent advances in small molecule microarrays: applications and technology Combinatorial Chemistry & High Throughput Screening. ,vol. 7, pp. 557- 564 ,(2004) , 10.2174/1386207043328427
Mahesh Uttamchandani, Shao Q. Yao, The expanding world of small molecule microarrays. Methods of Molecular Biology. ,vol. 1518, pp. 1- 17 ,(2010) , 10.1007/978-1-4939-6584-7_1
Haibin Shi, Mahesh Uttamchandani, Shao Q. Yao, A Method for Small Molecule Microarray-Based Screening for the Rapid Discovery of Affinity-Based Probes Methods in Molecular Biology. ,vol. 669, pp. 57- 68 ,(2010) , 10.1007/978-1-60761-845-4_5