Developing an appropriate strategy to assess genetic variability in plant germplasm collections

摘要: The value of molecular biology for monitoring the genetic status germplasm collections is subject to practical limitations. large number and variability accessions held usually dictates approach that can be employed. A quick, simple but reliable protocol must combined with an appropriate strategy handling sample sizes. In this study, ISSR-PCR was used reveal within between in a collection lupin germplasm. Pooling DNA from individuals found most assessing quantities plant material. Band profiles generated pools containing five were fully representative all constituent mix. Pools comprising 10 or 20 individuals, however, sometimes failed contain minor bands had been present only profile one individual. Variation observed different genotypes same accession. Routine large-scale screens are required assess diversity homogeneity Reading. It concluded 2–3 may sufficient represent similar collections.