Purification of phospholipase D by two-phase affinity extraction

作者: S Teotia , M.N Gupta

DOI: 10.1016/J.CHROMA.2003.10.104

关键词:

摘要: … An aqueous two-phase system of polyethylene glycol (PEG)–salt was used for purification … in aqueous two-phase system Aqueous two-phase separation using the PEG–salt system was …

参考文章(24)
Michael J. Dunn, Gel Electrophoresis of Proteins ,(2014)
Sunita Teotia, M.N Gupta, Reversibly soluble macroaffinity ligand in aqueous two-phase separation of enzymes Journal of Chromatography A. ,vol. 923, pp. 275- 280 ,(2001) , 10.1016/S0021-9673(01)00968-2
Aparna Sharma, Shweta Sharma, M.N. Gupta, Purification of wheat germ amylase by precipitation. Protein Expression and Purification. ,vol. 18, pp. 111- 114 ,(2000) , 10.1006/PREP.1999.1172
Sunita Teotia, S.K Khare, M.N Gupta, An efficient purification process for sweet potato beta-amylase by affinity precipitation with alginate. Enzyme and Microbial Technology. ,vol. 28, pp. 792- 795 ,(2001) , 10.1016/S0141-0229(01)00338-6
Ipsita Roy, Meryam Sardar, Munishwar N. Gupta, Exploiting unusual affinity of usual polysaccharides for separation of enzymes on fluidized beds. Enzyme and Microbial Technology. ,vol. 27, pp. 53- 65 ,(2000) , 10.1016/S0141-0229(00)00148-4
Sunita Teotia, R. Lata, M.N. Gupta, Free polymeric bioligands in aqueous two-phase affinity extractions of microbial xylanases and pullulanase. Protein Expression and Purification. ,vol. 22, pp. 484- 488 ,(2001) , 10.1006/PREP.2001.1466
Shweta Sharma, M.N. Gupta, Purification of Phospholipase D from Dacus carota by Three-Phase Partitioning and Its Characterization Protein Expression and Purification. ,vol. 21, pp. 310- 316 ,(2001) , 10.1006/PREP.2000.1357