Free polymeric bioligands in aqueous two-phase affinity extractions of microbial xylanases and pullulanase.
作者： Sunita Teotia , R. Lata , M.N. Gupta
关键词: Trichoderma reesei 、 Xylanase 、 Pullulanase 、 Aqueous two-phase system 、 Aspergillus niger 、 PEG ratio 、 Bacillus amyloliquefaciens 、 Polyethylene glycol 、 Chemistry 、 Chromatography
摘要: Two reversibly soluble-insoluble polymers (viz. Eudragit S-100 and alginate) were used as free macroaffinity bioligands in polyethylene glycol (PEG)/salt two-phase systems for separation of enzymes. Incorporation alginate the PEG phase led to considerable selectivity microbial xylanases pullulanase, respectively. Xylanase from Aspergillus niger was recovered 93% with 56-fold purification, whereas enzyme Trichoderma reesei Bacillus amyloliquefaciens obtained activity recovery (31-fold purification) 90% (32-fold purification), From acidopullulyticus 85% 44-fold purification obtained. The approach described here shows potential developing into a general use ligand affinity extraction.
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