Identification of allelic losses in benign, borderline, and invasive epithelial ovarian tumors and correlation with clinical outcome.

摘要: BACKGROUND The somatogenetic alterations that lead to the development of benign adenomas, borderline tumors, and invasive ovarian carcinomas are not well understood. This study investigated allelic losses in these three types tumors. METHODS Twelve genetic regions on chromosomes 2q, 5q, 6p, 6q, 9p, 11q, 17p, 17q, 18q, 22q were analyzed by polymerase chain reaction for loss heterozygosity (LOH). The was performed formalin fixed, paraffin embedded tissue samples from 72 carcinomas, 35 tumors malignancy, 25 adenomas. RESULTS LOH found only 8% adenomas (at 2q21 17p13) 11% 2q21, 5q21, 6p21, 17p13, 17q21). Allelic noted 77.7% all analyzed. frequency LOH 56% at a locus near 17p13 (TP53) 40.5% 17q21 (BRCA1). observed 30.4% informative cases 5q21 21.4% chromosome 18q21. chromosomal 2q21-22 9p21 had deletions frequencies 32.4% 25%, respectively. International Federation Gynecology Obstetrics stage presence correlated significantly with survival but independent predictors survival. Serous subtypes carcinoma more prone than nonserous tumors. CONCLUSIONS The results this suggest occurs occasionally whereas it is frequently carcinomas. Therfore, other events seem be involved early tumor development. However, multiple an indicator higher stages carcinoma. Cancer 1997; 80:1241-9. © 1997 American Society.