Reconstitution of male germline cell specification from mouse embryonic stem cells using defined factors in vitro.
作者： Na Li , Wentao Ma , Qiaoyan Shen , Mengfei Zhang , Zhaoyu Du
DOI: 10.1038/S41418-019-0280-2
关键词:
摘要: In vitro induction of functional haploid cells from embryonic stem (ESCs) has been reported by several groups. However, these reports either involve complex process with undefined factors or show low-induction efficiency. Here, we report complete meiosis in ESCs defined factors. were first induced into primordial germ cell-like cells, which further male germline including spermatogonial (SSCLCs) and spermatid-like cells. Importantly, the obtained SSCLCs as infertile mice sired healthy offspring via SSCLC transplantation. Further, found that eukaryotic translation initiation factor 2 subunit 3 structural gene Y-linked (Eif2s3y) was essential for spermatogenesis. Eif2s3y-overexpressing showed enhanced spermatogenesis vitro, demonstrated higher expression levels SSC-specific markers during process, improved reproductive ability recovery mice, increased efficiency cell induction. Our work provides a convenient efficient approach to obtain
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sci-hub.se 参考文章(29)
Sophie Mazeyrat, Noëmie Saut, Vladimir Grigoriev, Shantha K. Mahadevaiah, Obah A. Ojarikre, Áine Rattigan, Colin Bishop, Eva M. Eicher, Michael J. Mitchell, Paul S. Burgoyne, A Y-encoded subunit of the translation initiation factor Eif2 is essential for mouse spermatogenesis. Nature Genetics. ,vol. 29, pp. 49- 53 ,(2001) , 10.1038/NG717
Erna Magnúsdóttir, Sabine Dietmann, Kazuhiro Murakami, Ufuk Günesdogan, Fuchou Tang, Siqin Bao, Evangelia Diamanti, Kaiqin Lao, Berthold Gottgens, M Azim Surani, None, A tripartite transcription factor network regulates primordial germ cell specification in mice Nature Cell Biology. ,vol. 15, pp. 905- 915 ,(2013) , 10.1038/NCB2798
Fumio Nakaki, Katsuhiko Hayashi, Hiroshi Ohta, Kazuki Kurimoto, Yukihiro Yabuta, Mitinori Saitou, Induction of mouse germ-cell fate by transcription factors in vitro Nature. ,vol. 501, pp. 222- 226 ,(2013) , 10.1038/NATURE12417
C. Eguizabal, N. Montserrat, R. Vassena, M. Barragan, E. Garreta, L. Garcia-Quevedo, F. Vidal, A. Giorgetti, A. Veiga, J. C. Izpisua Belmonte, Complete Meiosis from Human Induced Pluripotent Stem Cells Stem Cells. ,vol. 29, pp. 1186- 1195 ,(2011) , 10.1002/STEM.672
Yohei Matsubara, Tomoko Kato, Kenichi Kashimada, Hiromitsu Tanaka, Zhou Zhi, Shizuko Ichinose, Shuki Mizutani, Tomohiro Morio, Tomoki Chiba, Yoshiaki Ito, Yumiko Saga, Shuji Takada, Hiroshi Asahara, TALEN-Mediated Gene Disruption on Y Chromosome Reveals Critical Role of EIF2S3Y in Mouse Spermatogenesis. Stem Cells and Development. ,vol. 24, pp. 1164- 1170 ,(2015) , 10.1089/SCD.2014.0466
Niels Geijsen, Melissa Horoschak, Kitai Kim, Joost Gribnau, Kevin Eggan, George Q. Daley, Derivation of embryonic germ cells and male gametes from embryonic stem cells Nature. ,vol. 427, pp. 148- 154 ,(2004) , 10.1038/NATURE02247
Mingzhao Li, Chao Liu, Haijing Zhu, Junwei Sun, Meng Yu, Zhiwei Niu, Weishuai Liu, Sha Peng, Jinlian Hua, Expression pattern of Boule in dairy goat testis and its function in promoting the meiosis in male germline stem cells (mGSCs) Journal of Cellular Biochemistry. ,vol. 114, pp. 294- 302 ,(2013) , 10.1002/JCB.24368
Yasuhiro Yamauchi, Jonathan M. Riel, Zoia Stoytcheva, Monika A. Ward, Two Y Genes Can Replace the Entire Y Chromosome for Assisted Reproduction in the Mouse Science. ,vol. 343, pp. 69- 72 ,(2014) , 10.1126/SCIENCE.1242544
Naoko Irie, Leehee Weinberger, Walfred W.C. Tang, Toshihiro Kobayashi, Sergey Viukov, Yair S. Manor, Sabine Dietmann, Jacob H. Hanna, M. Azim Surani, SOX17 Is a Critical Specifier of Human Primordial Germ Cell Fate Cell. ,vol. 160, pp. 253- 268 ,(2015) , 10.1016/J.CELL.2014.12.013
Sabrina Z. Jan, Geert Hamer, Sjoerd Repping, Dirk G. de Rooij, Ans M.M. van Pelt, Tinke L. Vormer, Molecular control of rodent spermatogenesis. Biochimica et Biophysica Acta. ,vol. 1822, pp. 1838- 1850 ,(2012) , 10.1016/J.BBADIS.2012.02.008