作者: Robert E Hurst , Kimberly D Kyker , Mikhail G Dozmorov , Nobuaki Takemori , Anil Singh
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摘要: The extracellular matrix can have a profound effect upon the phenotype of cancer cells. Previous work has shown that growth bladder cells on derived from normal basement membrane suppresses many malignant features are displayed when grown been modified by tumors. This was undertaken to investigate proteome-level changes as determined new commercially available proteome display involving 2-dimensional chromatography for different preparations modulate expression phenotype. Depending matrix, between 1300 and 2000 distinct peaks were detected two-dimensional chromatographic fractionation 2.1 – 4.4 mg total cellular protein. fractions eluting reversed-phase suitable mass spectrometric identification following only lyophilization trypsin digestion achieved approximately 10-fold higher sensitivity than obtained with gel-based separations. Abundant proteins unique one matrices identified spectrometry. Following concentration, 0.03 AU provided unambiguous protein components 10% sample analyzed, whereas 0.05 lower limit detection entire separated gel in-gel used. Although some homogeneous, others not, up 3 per fraction identified. Strong evidence post-translational modification noted. All 13 Matrigel related MYC pathway. system provides viable alternative electrophoresis proteomic biological systems. findings suggest importance