Proteome-level display by 2-dimensional chromatography of extracellular matrix-dependent modulation of the phenotype of bladder cancer cells.

作者: Robert E Hurst , Kimberly D Kyker , Mikhail G Dozmorov , Nobuaki Takemori , Anil Singh

DOI: 10.1186/1477-5956-4-13

关键词:

摘要: The extracellular matrix can have a profound effect upon the phenotype of cancer cells. Previous work has shown that growth bladder cells on derived from normal basement membrane suppresses many malignant features are displayed when grown been modified by tumors. This was undertaken to investigate proteome-level changes as determined new commercially available proteome display involving 2-dimensional chromatography for different preparations modulate expression phenotype. Depending matrix, between 1300 and 2000 distinct peaks were detected two-dimensional chromatographic fractionation 2.1 – 4.4 mg total cellular protein. fractions eluting reversed-phase suitable mass spectrometric identification following only lyophilization trypsin digestion achieved approximately 10-fold higher sensitivity than obtained with gel-based separations. Abundant proteins unique one matrices identified spectrometry. Following concentration, 0.03 AU provided unambiguous protein components 10% sample analyzed, whereas 0.05 lower limit detection entire separated gel in-gel used. Although some homogeneous, others not, up 3 per fraction identified. Strong evidence post-translational modification noted. All 13 Matrigel related MYC pathway. system provides viable alternative electrophoresis proteomic biological systems. findings suggest importance

参考文章(24)
Brian D Paasch, Yvonne S Lin, Seth Porter, Nishit B Modi, Timothy J Barder, Determination of Ro 48-3656 in rat plasma by reversed-phase high-performance liquid chromatography Comparison of 1.5-μm nonporous silica to 3.5-μm porous silica analytical columns Journal of Chromatography B: Biomedical Sciences and Applications. ,vol. 704, pp. 231- 242 ,(1997) , 10.1016/S0378-4347(97)00418-0
Mikhail G Dozmorov, Kimberly D Kyker, Ricardo Saban, Nicholas Knowlton, Igor Dozmorov, Michael B Centola, Robert E Hurst, Analysis of the interaction of extracellular matrix and phenotype of bladder cancer cells BMC Cancer. ,vol. 6, pp. 12- 12 ,(2006) , 10.1186/1471-2407-6-12
David G. Kaufman, Markku Seppälä, Julia T. Arnold, Bruce A. Lessey, Effect of normal endometrial stroma on growth and differentiation in Ishikawa endometrial adenocarcinoma cells. Cancer Research. ,vol. 62, pp. 79- 88 ,(2002)
Patricia Harnden, Ludwik K. Trejdosiewicz, Peter J. Selby, Jennifer Southgate, Barbara A. Smith, Wendy J. Kennedy, Identification of genes involved in human urothelial cell-matrix interactions: implications for the progression pathways of malignant urothelium. Cancer Research. ,vol. 61, pp. 1678- 1685 ,(2001)
David R. M. Graham, Steven T. Elliott, Jennifer E. Van Eyk, Broad-based proteomic strategies: a practical guide to proteomics and functional screening The Journal of Physiology. ,vol. 563, pp. 1- 9 ,(2005) , 10.1113/JPHYSIOL.2004.080341
Kimberly D. Kyker, Robert E. Hurst, George P. Hemstreet, Rebecca B. Bonner, Ron D. Bowditch, Matrix-dependent Plasticity of the Malignant Phenotype of Bladder Cancer Cells Anticancer Research. ,vol. 23, pp. 3119- 3128 ,(2003)
Jennifer A Tuxhorn, Truong D Dang, Megan J Smith, David R Rowley, Vincent C Smith, Gustavo E Ayala, Reactive stroma in human prostate cancer: induction of myofibroblast phenotype and extracellular matrix remodeling Clinical Cancer Research. ,vol. 8, pp. 2912- 2923 ,(2002)
David N. Perkins, Darryl J. C. Pappin, David M. Creasy, John S. Cottrell, Probability-based protein identification by searching sequence databases using mass spectrometry data. Electrophoresis. ,vol. 20, pp. 3551- 3567 ,(1999) , 10.1002/(SICI)1522-2683(19991201)20:18<3551::AID-ELPS3551>3.0.CO;2-2
Kamala P. Sundararaj, Rachel E. Wood, Suriyan Ponnusamy, Arelis M. Salas, Zdzislaw Szulc, Alicia Bielawska, Lina M. Obeid, Yusuf A. Hannun, Besim Ogretmen, Rapid Shortening of Telomere Length in Response to Ceramide Involves the Inhibition of Telomere Binding Activity of Nuclear Glyceraldehyde-3-phosphate Dehydrogenase Journal of Biological Chemistry. ,vol. 279, pp. 6152- 6162 ,(2004) , 10.1074/JBC.M310549200
Catherine Booth, Patricia Harnden, Peter J Selby, Jennifer Southgate, Towards defining roles and relationships for tenascin-C and TGFβ-1 in the normal and neoplastic urinary bladder The Journal of Pathology. ,vol. 198, pp. 359- 368 ,(2002) , 10.1002/PATH.1214