Genetic Instruction of Megakaryocytes and Platelets Derived from Human Induced Pluripotent Stem Cells for Studies of Integrin Regulation.

摘要: Platelets are small, anucleate cells that play oversized roles in hemostasis, immunity, and inflammation. An important mediator of platelet function is integrin αIIbβ3, which required for fibrinogen-dependent aggregation during hemostasis. This response dependent on conformational changes the induced by "inside-out" biochemical signals triggered agonists. In turn, fibrinogen binding to αIIbβ3 initiates "outside-in" mechanical regulate cytoskeleton help promote full secretory responses. Without a nucleus, there limited range experimental manipulations possible with human platelets study molecular basis signaling these primary cells. Consequently, many studies use genetic approaches rely heterologous expression systems or from gene-targeted mice, sometimes uncertain applicability platelets. chapter will detail method manipulation megakaryocytes derived pluripotent stem modeling functions potentially relevant