Antimutagenicity and mutagen-binding activation of mutagenic pyrolyzates by microorganisms isolated from japanese miso

摘要: The microbiological flora of miso, a traditional fermented food in Japan, were investigated. Bacteria, yeast and mould isolated identified as Pediococcus acidilactici, Leuconostoc paramesenteroides, Micrococcus halobius, Zygosaccharomyces rouxii Aspergillus sp. P acidilactici strains dominant bacteria miso. The binding antimutagenic activities all microbial towards mutagenic pyrolysates lyophilised cells the showed largest effect on 3-amino-l-methyl-[5H]pyrido[4,3-b]indole (Trp-P-2), but was less than yeast. Most tested had no effective activity against 2-amino-3-methyl imidazo[4,5-f] quinoline (IQ). Trp-P-2 effectively bound by non-mould IQ to much effective. Among tested, paramesenteroides No 28 indicated highest activity, not only Trp-P-2 also (30% capacity). As concentration increased, limits ability 23, Z 6 sp 1 650, 500 400μg per 5 mg respectively. The antimutagenicity for reduced autoclaving at 100°C min or 121°C 15 min, 6–20% 7–28%, respectively. unaffected autoclaving. The cell walls very high, being more 85 YO effective, it lower that cytoplasm.