Hypothesis: Artifacts, Including Spurious Chimeric RNAs with a Short Homologous Sequence, Caused by Consecutive Reverse Transcriptions and Endogenous Random Primers.
作者： Zhiyu Peng , Chengfu Yuan , Lucas Zellmer , Siqi Liu , Ningzhi Xu
DOI: 10.7150/JCA.11997
关键词:
摘要: Recent RNA-sequencing technology and associated bioinformatics have led to identification of tens thousands putative human chimeric RNAs, i.e. RNAs containing sequences from two different genes, most which are derived neighboring genes on the same chromosome. In this essay, we redefine “two genes” as those producing individual transcripts, point out known mechanisms for RNA formation, transcription a fusion gene or trans-splicing RNAs. By our definition, chimeras canonically-defined may either be technical artifacts cis-splicing products 5'- 3'-extended partner that is redefined herein an unannotated gene, whereas events rare in cells. Therefore, authentic result about 1,000 been identified hitherto. We propose hypothesis “consecutive reverse transcriptions (RTs)”, another RT reaction following previous one, how spurious especially short homologous sequence, generated during RT, wherein fragmented. also samples contain numerous DNA shreds can serve endogenous random primers ensuing polymerase chain reactions (PCR), creating RT-PCR.
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