Site-specific excision from RNA by RNase H and mixed-phosphate-backbone oligodeoxynucleotides.
作者： S. Agrawal , S. H. Mayrand , P. C. Zamecnik , T. Pederson
关键词:
摘要: Oligodeoxynucleotides containing phosphodiester or modified internucleoside linkages were investigated with respect to their ability be acted on by ribonuclease H activities present in a HeLa cell nuclear extract after hybridization complementary sequences RNA. nucleotides 2-14 of human U1 small RNA investigated. Extensive cleavage was observed the unmodified oligodeoxynucleotide and phosphorothioate analogue but not U1-complementary oligodeoxynucleotides methylphosphonate, phosphoro-N-morpholidate, phosphoro-N-butylamidate linkages. Additional experiments using 514-nucleotide-long substrate demonstrated capacity phosphodiester- phosphorothioate-linked (but ones linkages) serve as RNase targets when hybridized an internal site. Detailed comparisons revealed phosphodiester-linked more efficient than comparable oligomers action. Various pentadecamer test 2-6 consecutive flanked H-resistant methylphosphonate afforded precise "site-directed" excision within DNA.RNA hybrid. These results assort oligodeoxynucleotide-containing hybrids into H-sensitive -resistant classes also provide clues how makes contact DNA strand
core.ac.uk
harvard.edu
jstor.org 
sci-hub.se 参考文章(32)
J R Patton, W Habets, W J van Venrooij, T Pederson, U1 small nuclear ribonucleoprotein particle-specific proteins interact with the first and second stem-loops of U1 RNA, with the A protein binding directly to the RNA independently of the 70K and Sm proteins. Molecular and Cellular Biology. ,vol. 9, pp. 3360- 3368 ,(1989) , 10.1128/MCB.9.8.3360
J R Patton, R J Patterson, T Pederson, Reconstitution of the U1 small nuclear ribonucleoprotein particle. Molecular and Cellular Biology. ,vol. 7, pp. 4030- 4037 ,(1987) , 10.1128/MCB.7.11.4030
C. C. Smith, L. Aurelian, M. P. Reddy, P. S. Miller, P. O. Ts'o, Antiviral effect of an oligo(nucleoside methylphosphonate) complementary to the splice junction of herpes simplex virus type 1 immediate early pre-mRNAs 4 and 5. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 83, pp. 2787- 2791 ,(1986) , 10.1073/PNAS.83.9.2787
M. Matsukura, G. Zon, K. Shinozuka, M. Robert-Guroff, T. Shimada, C. A. Stein, H. Mitsuya, F. Wong-Staal, J. S. Cohen, S. Broder, Regulation of viral expression of human immunodeficiency virus in vitro by an antisense phosphorothioate oligodeoxynucleotide against rev (art/trs) in chronically infected cells. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 86, pp. 4244- 4248 ,(1989) , 10.1073/PNAS.86.11.4244
John Goodchild, Eddie Carroll, Jay R. Greenberg, Inhibition of rabbit β-Globin synthesis by complementary oligonucleotides: Identification of mRNA sites sensitive to inhibition Archives of Biochemistry and Biophysics. ,vol. 263, pp. 401- 409 ,(1988) , 10.1016/0003-9861(88)90652-2
Helen Donis-Keller, Site specific enzymatic cleavage of RNA Nucleic Acids Research. ,vol. 7, pp. 179- 192 ,(1979) , 10.1093/NAR/7.1.179
Angus I. Lamond, Brian Sproat, Ursula Ryder, Jörg Hamm, Probing the structure and function of U2 snRNP with antisense oligonucleotides made of 2'-OMe RNA. Cell. ,vol. 58, pp. 383- 390 ,(1989) , 10.1016/0092-8674(89)90852-0
P. C. Zamecnik, M. L. Stephenson, Inhibition of Rous sarcoma virus replication and cell transformation by a specific oligodeoxynucleotide Proceedings of the National Academy of Sciences of the United States of America. ,vol. 75, pp. 280- 284 ,(1978) , 10.1073/PNAS.75.1.280
J. D. Dignam, R. M. Lebovitz, R. G. Roeder, Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei Nucleic Acids Research. ,vol. 11, pp. 1475- 1489 ,(1983) , 10.1093/NAR/11.5.1475
Brenda L. Bass, Harold Weintraub, An unwinding activity that covalently modifies its double-stranded RNA substrate. Cell. ,vol. 55, pp. 1089- 1098 ,(1988) , 10.1016/0092-8674(88)90253-X