Multiwalled carbon nanotube-induced pulmonary inflammatory and fibrotic responses and genomic changes following aspiration exposure in mice: A 1-year postexposure study.

摘要: Pulmonary exposure to multiwalled carbon nanotubes (MWCNT) induces an inflammatory and rapid fibrotic response, although the long-term signaling mechanisms are unknown. The aim of this study was examine effects 1, 10, 40, or 80 μg MWCNT administered by pharyngeal aspiration on bronchoalveolar lavage (BAL) fluid for polymorphonuclear cell (PMN) infiltration, lactate dehydrogenase (LDH) activity, lung histopathology responses in mouse lungs 1 mo, 6 yr postexposure. Further, a 120-μg crocidolite asbestos group incorporated as positive control comparative purposes. Results showed that increased BAL LDH activity PMN infiltration dose-dependent manner at all three postexposure times. Asbestos elevated 3 times mo Pathological changes lung, presence asbestos, fibrosis were noted 40 asbestos-exposed mice To determine potential pathways involved with MWCNT-associated pathological comparison up- down-regulated gene expression determined tissue Exposure tended favor those immune responses, specifically T-cell whereas oxygen species production, electron transport, cancer. Data indicate biopersistent induce alterations similar but may reach these endpoints different mechanisms.