Effect of N-pyridinium positions of quaternized chitosan on transfection efficiency in gene delivery system.
作者： Warayuth Sajomsang , Pattarapond Gonil , Uracha Rungsardthong Ruktanonchai , Maleenart Petchsangsai , Praneet Opanasopit
DOI: 10.1016/J.CARBPOL.2013.12.077
关键词:
摘要: Methylated N-pyridylmethyl chitosan chlorides (M-PyMeChCs) with a similar total degree of quaternization (DQT) and molecular weight but different N-pyridinium positions were synthesized by reductive amination methylation, respectively. The effect on transfection efficiency cytotoxicity was investigated in human hepatoma (Huh7) cell lines. results revealed that M-PyMeChCs are able to form complete complex formation DNA since there is an N/P ratio 5. particle sizes M-PyMeChCs/DNA nanopolyplexes approximately 300 nm indicated positive charge. morphology these found be spherical shape which using the transmission electron microscopy (TEM). M4-PyMeChC/DNA showed highest vitro Huh7 cells at 20 compared M2-PyMeChC/DNA M3-PyMeChC/DNA nanopolyplexes. In comparison nanopolyplexes, lower cells. Our result demonstrated related cytotoxicity.
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