Estimation of genetic variation in green tiger prawn, Penaeus semisulcatus by using random amplified polymorphic DNA, inter simple sequence repeat and simple sequence repeat markers
作者： Hesham Abdallah Hassanien , Yousof Al‐Rashada
DOI: 10.1111/ARE.14040
关键词:
摘要: In this study, three molecular markers including random amplified polymorphic DNA (RAPD), inter simple sequence repeat (ISSR) and (SSR) were used to evaluate genetic variation of green tiger prawn Penaeus semisulcatus collected from two geographically isolated environments; located in the Manifa, Arabian Gulf, Saudi Arabia Ataka, Suez Egypt. Genetic parameters included percentage polymorphism (P%), effective alleles (Ne), Nei diversity (H) Shannon index (I), which calculated based on data. All marker systems distinguished P. various levels. The highest (91.30%) was obtained with SSR, followed by ISSR (82.26%) RAPD (62.04%), respectively. Our results indicate that SSR appeared be best suited assay for assessing between genotypes semisulcatus. present study indicated Manifa Ataka closely related. Moreover, analysis variability could require more than one DNA‐based techniques.
sci-hub.se 参考文章(31)
Ayman M. Sabry, Mohamed M. Hassan, Alaa A. Mohamed, Molecular Characterization of Some Popular Fish Species in Saudi Arabia International Journal of Applied Sciences and Biotechnology. ,vol. 3, pp. 359- 366 ,(2015) , 10.3126/IJASBT.V3I2.12747
G. Hulata, Genetic manipulations in aquaculture: a review of stock improvement by classical and modern technologies Genetica. ,vol. 111, pp. 155- 173 ,(2001) , 10.1023/A:1013776931796
C Callejas, M D Ochando, Phylogenetic relationships among Spanish Barbus species (Pisces, Cyprinidae) shown by RAPD markers. Heredity. ,vol. 89, pp. 36- 43 ,(2002) , 10.1038/SJ.HDY.6800091
Daud Siti Khalijah, N. Niamaimandi, B. Kiabi, A. Arshad, Saed Ross Cheroos, Population structure of green tiger prawn, Penaeus semisulcatus (De Haan) in Bushehr waters, Persian Gulf Iranian Journal of Fisheries Sciences. ,vol. 9, pp. 337- 341 ,(2010)
Joseph C Bagshaw, Michael A Buckholt, A novel satellite/microsatellite combination in the genome of the marine shrimp, Penaeus vannamei Gene. ,vol. 184, pp. 211- 214 ,(1997) , 10.1016/S0378-1119(96)00597-5
Azza A. El-Ganainy, Mohamed H. Yassien, The population biology of penaeid prawns in the Gulf of Suez, Red Sea, Egypt Marine Biology Research. ,vol. 8, pp. 405- 411 ,(2012) , 10.1080/17451000.2011.637564
P. Rajakumaran, B. Vaseeharan, R. Jayakumar, R. Chidambara, Conformation of phylogenetic relationship of Penaeidae shrimp based on morphometric and molecular investigations Cytology and Genetics. ,vol. 48, pp. 357- 363 ,(2014) , 10.3103/S0095452714060103
Padmanava Mohanty, Lakshman Sahoo, Bindu R Pillai, Pallipuram Jayasankar, Paramananda Das, Genetic divergence in Indian populations of M. rosenbergii using microsatellite markers Aquaculture Research. ,vol. 47, pp. 472- 481 ,(2016) , 10.1111/ARE.12508
W. S. Lakra, M. Goswami, Akhilesh Singh, Premsagar Mishra, N. Gayathri, N. S. Nagpure, Genetic characterization of Metapenaeus affinis (H. M. Edwards, 1837) using RAPD markers Molecular Biology Reports. ,vol. 37, pp. 3757- 3761 ,(2010) , 10.1007/S11033-010-0029-Y
Bavornlak Khamnamtong, Sirawut Klinbunga, Piamsak Menasveta, Species identification of five penaeid shrimps using PCR-RFLP and SSCP analyses of 16S ribosomal DNA. Journal of Biochemistry and Molecular Biology. ,vol. 38, pp. 491- 499 ,(2005) , 10.5483/BMBREP.2005.38.4.491