Development and application of different methods for the detection of Toxoplasma gondii in water.

摘要: Two methods, centrifugation and flocculation, were evaluated to determine their efficiencies of recovery Toxoplasma gondii oocysts from contaminated water samples. Demineralized tap replicates inoculated with high numbers sporulated or unsporulated T. (1 × 105 1 104 oocysts). The strain, age, concentration the seeded recorded. Oocysts recovered either by samples at various g values flocculation two coagulants, Fe2(SO4)3 Al2(SO4)3. rates determined final pellets phase-contrast microscopy. Sporulated more effectively Al2(SO4)3 (96.5% ± 21.7%) than (93.1% 8.1%) 2,073 (82.5% 6.8%). For oocysts, was successful (100.3% 26.9%) (90.4% 19.1%) 2,565 (97.2% 12.5%). infectivity confirmed seroconversion all mice 77 days postinfection. These data suggest that purified methods commonly used for waterborne pathogens retain after mechanical treatment are able induce infections in mammals. This is first step developing a systematic approach detection water.