Role of T cell subsets in the maternal-to-neonatal transmission of immunity against Trichinella spiralis during lactation in rats

摘要: Abstract We have previously demonstrated the maternal-to-neonatal transfer of immunity to T. spiralis during lactation and shown that antigen-specific T lymphocytes, when injected into mother or orally fed neonates, can mediate this transfer. To further analyze cell subsets involved in conferring protection, lymphocytes were isolated from mesenteric lymph nodes syngeneic donor rats infected 4–6 days earlier with spiralis. The cells incubated vitro either mouse-anti-rat OX8 W3/25 monoclonal antibody, “panned” on plates coated goat-anti-mouse Ig, non-adherent helper cytotoxic/suppressor harvested. 100 × 106 i.v. mothers once early again two prior challenging their pups (200 larvae) at 2 weeks age. This resulted significant passage suckling worm counts being 59% 73% control values 3 8 post-challenge (P