Assembly of Synapses: Biomimetic Assays to Control Neurexin/Neuroligin Interactions at the Neuronal Surface
作者： Magali Mondin , Béatrice Tessier , Olivier Thoumine
DOI: 10.1002/0471142301.NS0219S64
关键词:
摘要: The role of adhesion molecules in the assembly synapses nervous system is an important issue. To characterize neurexin/neuroligin complexes synapse differentiation, various imaging assays can be performed primary hippocampal cultures. First, to temporally control contact formation, biomimetic using microspheres coated with purified neurexin or antibody clusters that aggregate neurexin. These models are combined live fluorescence study dynamics accumulation post-synaptic components, including scaffolding and glutamate receptors. demonstrate AMPA receptors recruited nascent contacts through lateral diffusion, mobility neuronal membrane monitored by tracking individual quantum dots (QDs) conjugated antibodies against Experiments monitoring attachment detachment Nrx-coated QDs measure rates interaction also performed. Each these detailed this unit.
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