Tumor necrosis factor alpha induces upregulation of CXC-chemokine receptor type II expression and magnifies the proliferative activity of CXC-chemokines in human melanocytes.

摘要: The CXC-chemokines Groalpha and interleukin-8 (IL-8) are ligands for two different G protein-coupled receptors, named CXC-chemokine receptor I & II (CXCRI II). Both cytokines potent growth factors human melanoma cells, with only limited proliferative activity towards normal melanocytes. Here we analysed the influence of various on expression CXCRI CXC-chemokine-induced proliferation in Flow cytometric studies revealed no protein low CXCRII at cell surface Tumor necrosis factor alpha (TNFalpha) enhanced mRNA CXCRII, but did not CXCRI. A consequence TNFalpha-pretreatment melanocytes was a significant enhancement IL-8 Groalpha. This study implicates that TNFalpha magnifies biological by induction expression.