A comparative analysis of the time-dependent antiproliferative effects of daunorubicin and WP631.
作者： Silvia Villamarín , Sylvia Mansilla , Neus Ferrer-Miralles , Waldemar Priebe , José Portugal
DOI: 10.1046/J.1432-1033.2003.03442.X
关键词:
摘要: Jurkat T lymphocytes were treated with daunorubicin and WP631, a daunorubicin-based DNA binding agent, in experiments aimed to analyze cellular uptake of these drugs their effect on cell viability. WP631 was taken up more slowly than daunorubicin, but laser confocal microscopy spectrofluorometric quantification showed that the drug accumulated cells. Despite slow rate, antiproliferative capacity (measured as IC50 after 72-h continuous treatment) greater daunorubicin. The propensities promote apoptosis compared. Our results indicate major G2/M arrest followed, about 72 h treatment, by polyploidy mitotic (reproductive) death. In contrast, induced rapid response classic features apoptosis.
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