Sorting out the role of reactive oxygen species during plant programmed cell death induced by ultraviolet-C overexposure

摘要: Previous studies have reported that light is required for activating Arabidopsis programmed cell death (PCD) induced by ultraviolet-C (UV-C) overexposure, and a caspase-like protease cleaving the caspase-3 substrate Asp-Glu-Val-Asp (DEVDase activity) during this process. Our recent report has suggested quick burst of reactive oxygen species (ROS), which mainly derived from mitochondria chloroplasts, in dependent manner early stages UV-induced plant PCD. Concomitantly, undergo serious dysfunction including MTP loss changes distribution mobility, ultimately lead to apoptotic-cell death. Though some signaling molecules been elucidated type death, molecular mechanism about UV-induce PCD still poorly understood when comparing with study pathways involved animal apoptosis UV. By using mesophyll protoplasts as reference model, we begun shed on complexity pathway Recently tried real-time detect presence proteolytic activation, sort out key role ROS well further assess relationship between production activation apoptotic