Fungal enzymes for environmental purposes, a molecular biology challenge.
作者： Marcela Ayala , Michael A. Pickard , Rafael Vazquez-Duhalt
DOI: 10.1159/000121328
关键词:
摘要: In their capacity to transform xenobiotics and polluting compounds, fungal peroxidases use in the environmental field have a recognized important potential. However, both fundamental practical issues, such as enzyme stability availability, delayed development of industrial applications. Three main protein engineering challenges been identified: (1) Enhancement operational stability, specifically hydrogen peroxide case peroxidases. (2) Increase redox potential order widen substrate range. (3) Development heterologous expression production. The bottlenecks, advances strategies that proven successful are discussed.
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