Stromelysin-3 expression by mammary tumor-associated fibroblasts under in vitro breast cancer cell induction
作者： Chang Shu Wang , Bernard Têtu
DOI: 10.1002/IJC.10430
关键词:
摘要: To understand better the influence of host stromal phenotype on expression stromelysin-3 (ST3) in breast cancer, we have investigated ST3 by cells isolated from 9 different primary carcinomas. These tumor-associated fibroblasts were cocultivated with 3 epithelial cancer cell lines mammary origin (MDA-MB-231, SK-BR-3 and MCF-7), as well normal human (NME 184A1) keratinocytes, using both direct indirect coculture systems. was demonstrated situ hybridization immunocytochemistry. The results showed that cancer-specific. Indeed, induced malignant whereas no expressed under stimulation. weak or absent unstimulated fibroblasts. However, after cells, transcripts reappeared 8 cases observed only located close contact tumor cells. Under similar conditions same line stimulation, was, however, quite variable among these cases, reflecting difference protease sections original tumors. Tumor induction much more important cell-cell than stimulation not influenced addition basic fibroblast growth factor (bFGF) anti-bFGF to culture medium. Our suggest may significantly © 2002 Wiley-Liss, Inc.
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