Real-time monitoring primary cardiomyocyte adhesion based on electrochemical impedance spectroscopy and electrical cell-substrate impedance sensing.

摘要: The cell−substrate distance is a direct indicator of cell adhesion to extracellular matrix which indispensable in culture. A real-time monitoring approach can provide detailed profile adhesion, so that enables the detecting adhesion-related behavior. In this work, we report novel impedance-based method record cardiomyocyte, overcoming its inscrutability due primary Microfabricated biosensors are applied cardiomyocyte culture after characterizing cell-free system. Cyclic frequency scanning data cell-related impedance generated and automatically fit into equivalent circuit model, established using electrochemical spectroscopy. displayed as alteration normalized essential parameters for manual electric sensing calibration absolute distance. time course displays significant decline cell−subs...