No evidence for a placental microbiome in human pregnancies at term

摘要: Abstract BACKGROUND The placenta has an important role in the modulation of pregnancy immunity; however, there is no consensus regarding existence a placental microbiome healthy full-term pregnancies. OBJECTIVE To investigate and origin microbiome. STUDY DESIGN A cross-sectional study comparing samples (three layers tissue, amniotic fluid, vernix as well saliva, vaginal rectal samples) from two groups births: 50 women not labor with elective cesarean deliveries 26 deliveries. comparisons were performed using PCR amplification DNA sequencing techniques bacterial culture experiments. RESULTS There significant differences background characteristics between who delivered by those vaginally. Quantitative measurements content all three (qPCR 16S rRNA gene) did show any difference sample types negative controls. gene maternal side could differentiate bacteria tissue contamination laboratory reagents DNA. Probe-specific qPCR for taxa suspected to be present detect statistically groups. In cultures, significantly more observed than colonies revealed that most grew on plates genera typically found human skin; moreover, it placentas vaginally contained high prevalence common bacteria. Bacterial growth inhibition experiments indicated may facilitate growth. CONCLUSIONS We evidence support our 76 term pregnancies, which used Incidental findings species due or low-grade presence some locations; such do represent per se.