Human placental extract mediated inhibition of proteinase K: implications of heparin and glycoproteins in wound physiology.

摘要: Efficient debridement of the wound bed following removal microbial load prevents its progression into a chronic wound. Bacterial infection and excessive proteolysis characterize impaired healing therefore, their inhibition might restore disturbed equilibrium in process. Human placental extract exhibits reversible, non-competitive towards Proteinase K, protease, by stabilizing it against auto-digestion. Scattering fluorescence studies followed biochemical analysis indicated involvement glycan moiety. Surface plasmon resonance demonstrated specific interaction heparin with K having Kd μM range. Further, contains sequence motifs similar to other heparin-binding proteins. Molecular docking revealed presence clefts suitable for binding heparin-derived oligosaccharides. Comprehensive this inhibitory property partly explains efficacy curing wounds common bacterial infections. J. Cell. Physiol. 229: 1212–1223, 2014. © 2014 Wiley Periodicals, Inc.