Doxazosin nanoencapsulation improves its in vitro antiproliferative and anticlonogenic effects on breast cancer cells

摘要: Abstract Doxazosin has been evaluated for the treatment of several types cancer. Here, antitumor effect nanoencapsulated form doxazosin was in an vitro model breast cancer (MCF7 cell line). Doxazosin-loaded polymeric nanocapsules (DXZ-NC) were produced by interfacial deposition preformed polymer with homogeneous aspect, spherical shape, mean diameter about 130 nm, positive zeta potential (+5 mV), and encapsulation efficiency close to 35%. The Alamar Blue ® assay counting carried out assess viability number, respectively. Mechanism death Annexin/Propidium Iodide staining, while long-term response assessed using clonogenic assay. Nuclear morphometric analysis investigated NMA technique. A significant decrease clonogenicity observed after DXZ-NC when compared non-encapsulated drug. All treatments induced apoptosis as main mechanism toxicity. In conclusion, nanoencapsulation improved its effects MCF7 cells, without changing underlying This approach fundamental reduce ability remaining tumor cells new colonies treatment, potentially reducing risk recurrence.