Erythrocytes from cigarette smokers contain more glutathione and catalase and protect endothelial cells from hydrogen peroxide better than do erythrocytes from nonsmokers.

摘要: Recent observations regarding the ability of intracellular erythrocyte (RBC) antioxidants to decrease O2 metabolite-mediated injury lung tissues has prompted interest in RBC patients with disease. We found that from 14 healthy, age- and gender-matched cigarette smokers contained more (p less than 0.05) glutathione (6.3 +/- 0.4 microM/g Hgb versus 5.0 0.3 Hgb) catalase (249,533 8,307 units/g 222,617 7,180 did nonsmokers. In contrast, nonsmokers same activities peroxidase (21.4 1.2 20.4 5.5 Hgb). also protected bovine pulmonary artery endothelial cells culture hydrogen peroxide (H2O2) better 0.005) (52.1 6.1% protection 31.9 5.7% protection). The results suggest alterations may reflect exposure and/or affect susceptibility oxidant-induced injury.