Ruffling and locomotion: role in cell resistance to growth factor-induced proliferation.

摘要: It has long been known that the growth rate of cells in vitro can be retarded by providing substrates restricted area. Such experiments were performed with adhesive islets, made depositing metals onto agarose layers through templates various sizes. Since normal are unable to adhere agarose, they become confined metallic surface. Using such haptotactic we have studied role membrane ruffling and cell locomotion resistance AG1523 human fibroblasts factor-induced mitogenesis. Cells plated on small substrates, i.e., 2,150 microns 2 area, initially showed vigorous ruffling, which was suppressed 8 h after plating but had resumed again 12 h. In contrast, larger-size islets a rapid decline stabilization activity. When measured for single cultured it found increase linearly from areas 4,280 up 425,000 2. area needed saturate response approximately 50-fold larger than occupied cell, inhibition attributed part an interference locomotion. The implication provided positive input into control mechanisms subjected direct test evaluating effect nine polypeptide factors motility serum-starved cells. All except TGF-beta 1 stimulated movement. Finally, mitogenic [3H]thymidine incorporation proportional motile activities, as quantitatively assayed. We conclude suppression is factor