QUANTITATIVE SPECTROFLUORIMETRY‐THE FLUORESCENCE QUANTUM YIELD OF QUININE SULFATE
作者： J. W. Eastman
DOI: 10.1111/J.1751-1097.1967.TB08790.X
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摘要: Abstract— By idealizing the design of a typical spectrofluorimeter that measures fluorescence from dilute solutions, we are able to derive an equation predicts dependence observable amplitude on concentration solute and several instrumental parameters. The result derivation, function, is used together with measurements rhodamine B colloidal silica scattering calibrate spectrofluorimeter. The function calibration data determine absolute quantum yield quinine sulfate in 0–1 N H2 So4. results, O250=0.582 excitation at 250 mμ, adn O350=0.577 350mμ. agree well previous evaluation Melhuish, O345=0.546.
sci-hub.se 参考文章(19)
F. W. J. Teale, G. Weber, Ultraviolet fluorescence of the aromatic amino acids Biochemical Journal. ,vol. 65, pp. 476- 482 ,(1957) , 10.1042/BJ0650476
W. H. Melhuish, Distribution of Fluorescence from a Disk-Shaped Cuvette* Journal of the Optical Society of America. ,vol. 51, pp. 278- 279 ,(1961) , 10.1364/JOSA.51.000278
J. M. Price, M. Kaihara, H. K. Howerton, Influence of Scattering on Fluorescence Spectra of Dilute Solutions Obtained with the Aminco–Bowman Spectrophotofluorometer Applied Optics. ,vol. 1, pp. 521- 533 ,(1962) , 10.1364/AO.1.000521
Gj. Deželić, J. P. Kratohvil, Determination of size of small particles by light scattering. experiments on ludox colloidal silica Colloid and Polymer Science. ,vol. 173, pp. 38- 48 ,(1960) , 10.1007/BF01513625
C. A. Parker, W. T. Rees, Correction of fluorescence spectra and measurement of fluorescence quantum efficiency The Analyst. ,vol. 85, pp. 587- 600 ,(1960) , 10.1039/AN9608500587
H. V. Drushel, A. L. Sommers, R. C. Cox, Correction of Luminescence Spectra and Calculation of Quantum Efficiencies Using Computer Techniques. Analytical Chemistry. ,vol. 35, pp. 2166- 2172 ,(1963) , 10.1021/AC60206A053
R. J. Argauer, C. E. White, Fluorescent Compounds for Calibration of Excitation and Emission Units of Spectrofluorometer. Analytical Chemistry. ,vol. 36, pp. 368- 371 ,(1964) , 10.1021/AC60208A038
P. Rosen, G. M. Edelman, Versatile and Stable Recording Spectrofluorometer for the Measurement of Corrected Spectra Review of Scientific Instruments. ,vol. 36, pp. 809- 815 ,(1965) , 10.1063/1.1719709
Paul Doty, Robert F. Steiner, Light Scattering and Spectrophotometry of Colloidal Solutions The Journal of Chemical Physics. ,vol. 18, pp. 1211- 1220 ,(1950) , 10.1063/1.1747913
H. E. Johns, A. M. Rauth, THEORY AND DESIGN OF HIGH INTENSITY U.V MONOCHROMATORS FOR PHOTOBIOLOGY AND PHOTOCHEMISTRY Photochemistry and Photobiology. ,vol. 4, pp. 673- 692 ,(1965) , 10.1111/J.1751-1097.1965.TB07910.X