Rapid endocytosis of interleukin-15 by cerebral endothelia

摘要: Interleukin (IL)-15 receptors are present in the cerebral endothelia composing blood-brain barrier where they show robust up-regulation by neuroinflammation. To determine how IL15 receptor subunits participate endocytosis and intracellular trafficking of IL15, we performed confocal microscopic imaging radioactive tracer uptake assays primary brain microvessel endothelial cells related cell lines transfected with modulatory molecules. By immunostaining co-localization studies organelle markers, showed that was rapidly endocytosed via lipid rafts directed to diverse pathways. During course trafficking, Alexa dye-conjugated partially co-localized both specific IL15Rα co-receptor IL2Rγ. However, deletion one had only a minor effect slowing when from knockout mice were compared those wildtype mice. trafficked early, recycling, late endosomes, Golgi, lysosomes. The diffuse distribution suggests activates multiple signaling events.