Subcellular localization and internalization of the four human leptin receptor isoforms.

作者: Valarie A. Barr , Kimberly Lane , Simeon I. Taylor

DOI: 10.1074/JBC.274.30.21416

关键词:

摘要: There are four known isoforms of the human leptin receptor (HLR) with different C-terminal cytoplasmic domains (designated by number unique amino acids). In cells expressing HLR-5, -15, or -274, 15–25% binding sites were located at plasma membrane. contrast, in HLR-67, only 5% total Immunofluorescent microscopy showed that all partially co-localized calnexin and β-COP, markers endoplasmic reticulum Golgi, respectively. All also detected an unidentified punctate compartment. internalized via clathrin-mediated endocytosis, but rates. After 20 min 37 °C, 45% a bound cohort labeled ligand had been HLR-15, 30% 25% HLR-274, 15% HLR-5. Degradation occurred lysosomes. Overnight exposure to down-regulated isoforms, variable extent. HLR-274 displayed greatest down-regulation appeared reach lysosomes more quickly than other isoforms. The faster degradation may help terminate signaling.

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