Interactions between non-barbiturate injectable anesthetics and conventional antiepileptic drugs in the maximal electroshock test in mice—an isobolographic analysis

摘要: The aim of this study was the isobolographic evaluation interactions between three non-barbiturate intravenous anesthetics and conventional antiepileptic drugs in maximal electroshock-induced seizures mice. Electroconvulsions were produced by means an alternating current (ear-clip electrodes, 0.2-s stimulus duration, tonic hindlimb extension taken as endpoint). Adverse effects evaluated chimney test (motor performance) passive avoidance task (long-term memory). Plasma levels measured immunofluorescence. Obtained results indicate that ketamine acts synergistically with valproate carbamazepine. Also combinations propofol or phenobarbital led to synergistic interactions. An antagonism found etomidate carbamazepine phenobarbital. On other hand, diphenylhydantoin injectable proved be additive. only exception combination (1:3). Pharmacokinetic phenomena do not seem interfere observed interactions, since none influenced free plasma concentrations drugs. Referring undesired effects, impaired long-term memory. Although did disturbed motor coordination, it enhanced impairment caused diphenylhydantoin. Results present suggest needs avoided epileptic patients due a possibility negative some seizure precipitation.