p53 and K-ras gene mutations in carcinoma of the rectum among Finnish women

摘要: Aims/Background—The aim of this study was to identify p53 and K-ras gene mutations in carcinoma the rectum among Finnish women. Mutation patterns might give clues aetiological factors when comparisons are made with other human tumours. Methods—Of 134 women rectum, paraffin wax embedded specimens tumour tissue were obtained from 118 patients. Genomic DNA extracted, exons 4–8 codons 12/13 61 amplified, analysed for by single strand conformation polymorphism direct sequencing. The production proteins studied immunohistochemistry. Results—The overall crude frequency 35% but true appears be higher (up 56%). In gene, mutation (15%) significantly lower than that reported colon cancer. increased patient age. a high proportion patients (14%) rectal tumours contained small subclones cells displayed extremely rare at 110 232 gene. Hot spot codon 175 less common cancer colon. Conclusions—Rectal has characteristics genes uncommon tumours, including colon. A biological explanation these findings is not clear present, associated an unidentified genetic factor Finland.