Complementation of placental defects and embryonic lethality by trophoblast-specific lentiviral gene transfer
作者： Yuka Okada , Yuko Ueshin , Ayako Isotani , Tomoko Saito-Fujita , Hisako Nakashima
DOI: 10.1038/NBT1280
关键词:
摘要: Placental dysfunction underlies many complications during pregnancy, and better understanding of gene function placentation could have considerable clinical relevance. However, the lack a facile method for placenta-specific manipulation has hampered investigation placental organogenesis treatment dysfunction. We showed previously that transduction fertilized mouse eggs with lentiviral vectors leads to transgene expression in both fetus placenta. Here we report incorporation by blastocysts after removal zona pellucida. All placentas analyzed, but none fetuses, were transgenic. Application this substantially rescued mice deficient Ets2, Mapk14 (also known as p38alpha) Mapk1 Erk2) from embryonic lethality caused defects. Ectopic Mapk11 also complemented deficiency placentation.
nature.com
elsevier.com
sci-hub.se 参考文章(29)
Antonia Follenzi, Laurie E. Ailles, Silvia Bakovic, Massimo Geuna, Luigi Naldini, Gene transfer by lentiviral vectors is limited by nuclear translocation and rescued by HIV-1 pol sequences. Nature Genetics. ,vol. 25, pp. 217- 222 ,(2000) , 10.1038/76095
Janet Rossant, James C. Cross, Placental development: Lessons from mouse mutants Nature Reviews Genetics. ,vol. 2, pp. 538- 548 ,(2001) , 10.1038/35080570
Ralf H. Adams, Almudena Porras, Gema Alonso, Margaret Jones, Kristina Vintersten, Simona Panelli, Amparo Valladares, Lidia Perez, Rüdiger Klein, Angel R. Nebreda, Essential Role of p38α MAP Kinase in Placental but Not Embryonic Cardiovascular Development Molecular Cell. ,vol. 6, pp. 109- 116 ,(2000) , 10.1016/S1097-2765(05)00014-6
Masahito Ikawa, Nobushige Tanaka, Winston W.-Y Kao, Inder M Verma, Generation of transgenic mice using lentiviral vectors: a novel preclinical assessment of lentiviral vectors for gene therapy Molecular Therapy. ,vol. 8, pp. 666- 673 ,(2003) , 10.1016/S1525-0016(03)00240-5
E.C. Curnow, I.M. Gunn, A.O. Trounson, Electrofusion of two-cell bovine embryos for the production of tetraploid blastocysts in vitro. Molecular Reproduction and Development. ,vol. 56, pp. 372- 377 ,(2000) , 10.1002/1098-2795(200007)56:3<372::AID-MRD7>3.0.CO;2-W
J Han, J. Lee, L Bibbs, R. Ulevitch, A MAP kinase targeted by endotoxin and hyperosmolarity in mammalian cells Science. ,vol. 265, pp. 808- 811 ,(1994) , 10.1126/SCIENCE.7914033
Naoya Hatano, Yoshiko Mori, Masatsugu Oh-hora, Atsushi Kosugi, Takahiko Fujikawa, Naoya Nakai, Hitoshi Niwa, Jun-ichi Miyazaki, Toshiyuki Hamaoka, Masato Ogata, Essential role for ERK2 mitogen-activated protein kinase in placental development. Genes to Cells. ,vol. 8, pp. 847- 856 ,(2003) , 10.1046/J.1365-2443.2003.00680.X
Kumiko Tamura, Tatsuhiko Sudo, Uwe Senftleben, Agnes M Dadak, Randall Johnson, Michael Karin, Requirement for p38α in Erythropoietin Expression Cell. ,vol. 102, pp. 221- 231 ,(2000) , 10.1016/S0092-8674(00)00027-1
K. Rygaard, Agnès Revol, Dolores Esquivel-Escobedo, Bjarne L. Beck, H. A. Barrera-Saldaña, Absence of human placental lactogen and placental growth hormone (HGH-V) during pregnancy: PCR analysis of the deletion. Human Genetics. ,vol. 102, pp. 87- 92 ,(1998) , 10.1007/S004390050658
John C. Lee, Jeffrey T. Laydon, Peter C. McDonnell, Timothy F. Gallagher, Sanjay Kumar, David Green, Dean McNulty, Mary Jane Blumenthal, J. Richard Keys, Scott W. Land vatter, James E. Strickler, Megan M. McLaughlin, Ivo R. Siemens, Seth M. Fisher, George P. Livi, John R. White, Jerry L. Adams, Peter R. Young, A protein kinase involved in the regulation of inflammatory cytokine biosynthesis. Nature. ,vol. 372, pp. 739- 746 ,(1994) , 10.1038/372739A0