Enhanced in vivo gene transfer into the placenta using RGD fiber-mutant adenovirus vector
作者： Kazufumi Katayama , Rie Furuki , Hideaki Yokoyama , Makoto Kaneko , Masashi Tachibana
DOI: 10.1016/J.BIOMATERIALS.2011.02.038
关键词:
摘要: Among viral vectors, the fiber-mutant adenovirus vector carrying Arg-Gly-Asp (RGD) peptide sequence (Ad-RGD) seems to have potential for both clinical gene therapy and basic research. As a part of thorough evaluation Ad-RGD in preclinical studies, we designed an experiment investigate detail distribution compared with conventional (WT-Ad) pregnant mice. Surprisingly, had substantial placental tropism, at 10–100 times that WT-Ad. Transgene expression was sustained least 7 days, expressing firefly luciferase or red fluorescent protein has so far caused no dysfunction leading fetal death. showed high levels of transduction efficiency vitro-differentiated trophoblast stem cells, which higher αvβ3 integrin than undifferentiated cells observed. Our results suggest use another RGD-mediated targeting strategy holds promise drug delivery placenta.
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