Specific binding of prostaglandin D2 to rat brain synaptic membrane. Occurrence, properties, and distribution.

摘要: Prostaglandin (PG) D2 bound specifically to a particulate fraction rich in the synaptic membrane of rat brain. The binding was dependent on time and temperature, equilibrium being reached after 5 min at 37 degrees C. specific constituted about 70% total C, 55% 0 maximal obtained presence 100 mM sodium ion pH 8. dissociation constant concentration sites as determined by Scatchard analysis were 28 +/- 7 nM 0.45 pmol/mg protein (n = 3), respectively. Hill coefficient 1.15, indicating single entity no cooperativity. highly for PGD2; Ki values PGD1 PGF2 alpha 523 693 nM, Other PGs including 13,14-dihydro-15-keto-PGD2, an inactive metabolite PGD2, had 150- 1000-fold lower affinities than PGD2. inhibited boiling or treatment with proteases, phospholipases, beta-galactosidase. activity PGD2 highest pituitary gland, followed hypothalamus olfactory bulb od brain, this pattern almost parallel that cytosolic NADP-linked dehydrogenase activity. results suggest plays significant role these regions