Primary culture of glial cells from mouse sympathetic cervical ganglion: A valuable tool for studying glial cell biology

摘要: Central nervous system glial cells as astrocytes and microglia have been investigated in vitro many intracellular pathways clarified upon various stimuli. Peripheral cells, however, are not deeply despite its importance role inflammatory neurodegenerative diseases. Based on our previous experience of culturing neuronal objective was to standardize morphologically characterize a primary culture mouse superior cervical ganglion order obtain useful tool study peripheral cell biology. Superior ganglia from neonatal C57BL6 mice were enzymatically mechanically dissociated plated diluted Matrigel coated wells final concentration 10,000 cells/well. Five 8 days post plating, cultures fixed for morphological immunocytochemical characterization. Glial showed flat irregular shape, two or three long cytoplasm processes, round, oval shaped nuclei, with regular outline. Cell proliferation mitosis detected both qualitative quantitatively. able maintain their phenotype model including immunoreactivity against marker GFAP. This is the first description characterization sympathetic culture. work discusses uses limitations aspects