The comparative effect of busulphan ('Myleran') and aminochlorambucil on haemopoietic colony forming units in the rat.

摘要: Using the spleen colony assay technique, it has been shown that busulphan (‘Myleran’) in a dose of 10-2 g/kg (1/2 LD50), causes marked and prolonged depression (over 90%) number forming units per femur (CFU/femur). This is apparent before there any reduction total cell count maximal 2–4 days after an intraperitoneal (i.p.) injection drug. It then followed by steady recovery, normal values being reached about 20 days. In contrast, aminochlorambucil (2·5 x 10-3) = 1/2 LD50) although producing rapid fall marrow cellularity no discernable effect on CFU/femur. If, however, CFUs first induced and, allowing time for 50% recovery (13 days), now given, further severe CFUs/femur occurs resulting considerable prolongation neutropenia observed blood. The possible implications this mode action these two drugs, chemotherapy leukaemia, are discussed.