Somatic embryogenesis from seed explants of Abies alba
作者： Thomas Geier , Gerhard Reuther , Astrid Schuller
DOI: 10.1007/BF00042281
关键词:
摘要: Megagametophytes of Abies alba containing the immature embryos were dissected from seed coats and divided by longitudinal transverse sections. They placed with cut surface down on modified Schenk & Hildebrandt medium 50 mgl-1 myo-inositol 2% sucrose, supplemented 1 N6-benzyladenine (BAP). An embryogenic type callus proliferated after one month culture. Closer examination revealed presence structures resembling early stages embryogenesis as well single elongated, vacuolated cells clusters dense cytoplasm. Under appropriate conditions, some somatic elongated formed cotyledons.
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sci-hub.se 参考文章(12)
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